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Effect of chronic exposure to ammonia on alterations of proteins and immunoglobulins in sea bass (Dicentrarchus labrax) serum ArchiMer
Coeurdacier, Jean-luc; Dutto, Gilbert.
The relationship between serum protein, immunoglobulin concentrations and protein molecular weight profile (PMWP) alterations of sea bass (131.3 +/- 4.3 g) reared in sea water with sublethal concentrations of ammonia was studied over two periods totalling 116 days. During the exposure period (62 days) the first group (group 1) lived in sea water with 0.204 mg.L-1 unionized ammonia nitrogen equivalent to 12 % of the lethal concentration for 50 % of a population exposed for 96 h to ammonia (96-h LC50), whilst the second group (group 2) lived in sea water with 0.340 mg.L-1 UIA-N equivalent to 20 % of the 96-h LC50 of ammonia. Then, the two groups were left for a recovery period (54 days) in the same water as the control group (group 0). The determination of...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Fish culture; Proteins; Immunoglobulin; Sublethal toxicity; Ammonia; Dicentrarchus labrax; Poisson d'élevage; Protéine; Immunoglobuline; Toxicité sublétale; Ammoniaque.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-858.pdf
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Le système immunitaire du bar Dicentrarchus labrax. Immunoglobulines, cellules productrices d'immunoglobulines, ontogenèse applications à une pathologie virale néonatale ArchiMer
Breuil, Gilles.
The sea bass, Dicentrarchus labrax, has been subjected to intensive aquaculture on the Mediterranean cost for ten years. Marine fish hatcheries are key factors in the aquaculture field, and face different pathologies, especially one of viral origin (nodavirus) that could challenge their feasibility. Consequently, we have tried to provide some data on larvae and spawning stocks. Firstly, we have studied the immunoglobulins, components of the specific humoral immunity, and their role as antibodies. On adult basses, these immunoglobulins are tetrameric molecules with high molecular weight (850 kD), made of heavy (H) - 80 kD and light (L) - 26 kD chains. In the eggs, simplest forms such as monomeric or dimeric are brought to the fore; they are located under...
Tipo: Text Palavras-chave: Egg; Cytometry; Immunity system; Larvae; Immunoglobulin; Nodavirus; Dicentrarchus labrax; Hatchery; Ontogenese; Cytometrie; Immunoglobuline; Nodavirus; Oeuf; Larve; Nurserie; Dicentrarchus labrax.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/these-1265.pdf
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Recherche et caractérisation d'immunoglobulines dans les oeufs du loup (Dicentrarchus labrax) ArchiMer
Pepin, Jean-francois; Breuil, Gilles; Rigobert, P; Romestand, Bernard.
The occurrence of maternal immunity transfer in Sea bass (Dicentrarchus labrax) was investigated using ELISA and Western blot technics. In order to determine the possible presence of immunoglobulin (Ig), egg proteins obtained from fertilized eggs were analyzed. After several extraction protocol assays (precipitation, chromatography), monoclonal and polyclonal antibody probes specific to Sea bass Ig allowed to confirm the presence of an immunoglobulin-like protein in eggs with very low level. Biochemical characterization was carried out using SDS-PAGE. Results indicated that egg Ig had a molecular weight of 206 kDa. They were smaller than seric IgM (800 kDa). The heavy (H) and light (L) chains of the egg Ig and of the seric IgM had similar molecular weight,...
Tipo: Text Palavras-chave: Immunoglobuline; Transfert immunité maternelle; ELISA; Œuf; Poisson; Immunoglobulin; Maternal immunity transfer; ELISA; Eggs; Fish.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00272/38275/36458.pdf
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